##  [PCR Calculator](/thermal-wizard/pcr-calculator)

## PCR Calculator

[General Cooling Calculator](/thermal-wizard/qc-cooling-selector-home)[Device Cooling Calculator](/thermal-wizard/device-cooling-calculator)[Enclosure Cooling Calculator](/thermal-wizard/enclosure-cooling-calculator) 

[PCR Calculator](/thermal-wizard/pcr-calculator)[Air Cooling Calculator](/thermal-wizard/air-cooling-calculator)[Liquid Cooling Calculator](/thermal-wizard/liquid-cooling-calculator)

  Choose an Example or Complete the Requirements...

[![](/thermal-wizard/images/pcr-block-96.png)
PCR 96 Well Block](#top)

[![](/thermal-wizard/images/pcr-block-384.png)
PCR 384 Well Block](#top)

[![](/thermal-wizard/images/pcr-block-192.png)
PCR 192 Well Block](#top)

[![](/thermal-wizard/images/pcr-block-2.png)
PCR 2 Well Block](#top)

![Device Cooling Input Screen](/thermal-wizard/images/thermal-cycle-bkg.png)

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Dimensions

Length
  mm

Width
  mm

Height
  mm

---

Temperatures

Ambient Temperature (Tamb)
  °C

Elongation Temperature (Telg)
  °C

Denaturization Temperature (Tden)
  °C

Annealing Temperature (Tann)
  °C

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Ramp Rates

Maximum Heating Ramp Rate (Telg->Tden)
  °C/s

Maximum Cooling Ramp Rate (Tden->Tann)
  °C/s

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Sample Vials

Number of Vials

Select Vial Size
  0.1 mL 0.2 mL Thin-wall 0.2 mL 0.5 mL 1.5 mL 2 mL 10 mL

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Vial Material Properties

  Polypropylene Polyethylene terephthalate (PET) Polymethylpentene (TPX) Borosilicate Glass Other Material  Density:  kg/m3 Specific Heat:  J/kg*K

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Block Material Properties

  Al 100% Filled Al 80% Filled Al 60% Filled Al 50% Filled Al 40% Filled Brass Copper Gold Silver Stainless Steel Titanium Other Material  Density:  kg/m3   Specific Heat:  J/kg*K

Calculation Results...

Watts

BTU/hr

mm

in

°C

°F

 **Total Surface Area of PCR Block:**

 **PCR Block Volume (Vblock):**

 **Total Vial Volume (Vvial):**

 **Total Sample Volume (Vsample):**

 **Total Volume (Vblock + Vvial + Vsample):**

 **Total Envelope Volume (L x W x H):**

 **Passive Heat Loss (Annealing -> Ambient):**

 **Passive Heat Loss (Elongation -> Ambient):**

 **Passive Heat Loss (Denaturization -> Ambient):**

 **Heat to Add: Elongation -> Denaturization**

 **Heat to Remove: Denaturization -> Annealing**

 **Heating Power Required (Qh):**

 **Cooling Power Required (Qc):**

**Original URL:** https://tark-solutions.com/thermal-wizard/pcr-calculator